That's a good question. When we think about life, we must realize that the autonomous factories that use robots to collect raw materials can also be things that can fill the description of life. In that case, life is the living or non-living creature that can multiply and defend itself. Robot factories can operate quite independently, and their intelligence level is not probably higher than some cells or bacteria.
In that case, those independently operating factories can be remnants of past civilizations. But the thing is that the robots that have genetically cloned living brains can be as intelligent as humans or even more intelligent than we are. In those cases large number of living brains control the spacecraft that involve Von Neumann technology. And they can make copies of themselves.
So are those systems living or dead? Those brains need nutrients anyway. And that means those systems can be an ultimate hybridization. Between machines and living creatures. That kind of android can be extremely large and it can operate independently. The alien civilizations can move in space using radio waves. In those cases, the alien civilization sends the robot factory to another planet. And then it sends its genetic code to that factory. Then the AI creates synthetic DNA. And then it creates the new creature connecting that alien DNA with those planets' flora and fauna.
The life can continue as long as there is energy. The civilization can survive from the big silence or big crunch transforming it in the form of nanotechnology in the new universe. In that case, the nanomachine must travel billions of years, and then it must find the planet where there are living organisms. Then it must hybridize its DNA to those creatures.
Or it must transfer some crystals that can receive the wave movement that is the civilization's EEG code to those crystals that start to stress those creatures' nervous systems. But that thing could be a very difficult operation. And it requires the existence of a multiverse.
But can civilization move outside the universe? Moving outside the universe is not difficult. The system must use a solar sail and detonate antimatter behind it. When antimatter detonates between the craft and that shield it pulls the craft ahead. An artificial black hole or some kind of thermal pump can pull energy from around the craft into it. And that denies material turn into energy.
Life can be possible in the universe as long as there is energy, that creatures can use. Civilization can create rings around black holes, and those rings can harvest the last energy waves in the universe. But that thing means that when black holes are vaporizing that's the ultimate end.
What if Phoenix Universe is true? In that theory, the universe is born again and again. If the phoenix universe is true, the civilization can live until the next universe forms.
But those black holes vaporization creates shockwaves around the universe. Crossing shockwaves can form new Schwinger effect and wave-particle duality can form new particles. Or antimatter-matter particle pairs that send wave movement around emptiness. There are more and more crossings. And there is forming new particle-antiparticle pairs. Finally, that entirety forms a great black hole, and then another universe is born.
https://bigthink.com/starts-with-a-bang/how-long-life-persist-universe/
https://listverse.com/2019/03/19/10-realistic-designs-for-interstellar-spaceships/
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